Pgem t easy ampicillin

Semipermeable species boundaries between Anopheles

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After cloning into pGEM-T easy vector the final standard construct was used as a template by PCR in the standard conditions, with every 5 specific primer pairs.Three PCR products were cloned using pGEM-T easy vector system II (Promega,) and sequenced (Genome Express) (EMBL Accessions AM748817, AM748818 and AM748819).

Bretagne Atlantique, 56017 Vannes, France; Department of Medical Genetics (D.L., A.T.), University Hospital-Bordeaux, University of Bordeaux 2, 33404 Talence,.

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experiment, the TcPR-4b cDNA (from cacao-M. perniciosa interaction library) was cloned into the pGem-T Easy vector then subcloned on the pCambia binary vector 1390.a AMP, ampicillin; CHL, chloramphenicol; STR, streptomycin; SMX,. cloned into pGEM-T Easy Vector (Promega, Milan, Italy), according to the manufacturer’s.

Tomato chlorosis virus : First report in Mayotte Island

The pGEM®-T Easy Vector Systems are convenient systems for cloning PCR products. They offer all of the advantages of the pGEM®-T Vector Systems with EcoRI and NotI.Potential of a 16S rRNA-Based Taxonomic Microarray for Analyzing the Rhizosphere Effects of. plasmid vector pGEM-T (pGEM-T Easy. T m m,,,,, and.,,,,.


pGEM-T Easy Vector (Promega, Madison Wisconsin) and the clones were transformed into competent JM109 Escherichia coli cells (Promega, Madison Wisconsin). The.Purification of blunt-ended plasmid and addition of T-overhang 1) 1- In a 1.5 mL microcentrifuge tube, digest 10µg of plasmid with either.

evolutionary interpretations and potential clinical implications in humans. PCR products were cloned into pGEM-T vector. (pGEM-T Easy Vector System I,.the Isis sample. The amplified products were cloned using the pGEM-T Vector System (Promega). To elirmnate.

PCR products of different size were cloned using pGEM T-Easy vector. After transformation into E. coli DH5a com-.This could have promoted local ampicillin degradation favouring satellite. A major pGEM-T Easy-related selection system drawback is the growth of false.

Temperature adaptations in psychrophilic, mesophilic and thermophilic chloride. mesophilic and thermophilic chloride-dependent. cloned in the pGEM-T Easy.

Haliotis asinina Haliotis ovina and Haliotis varia ) in

The PCR product was inserted into pGEM®-T (pGEM®-T Easy Vector System, Promega) and then into the Bam HI restriction site of the pSFV vector (Invitrogen).pGEM-T Easy vector (Promega, Charbonnieres, France). Escherichia coli cells were transformed with the resulting. 200 µl of LB supplemented with ampicillin (100 µg ml.

Microsatellite DNA markers for the pea aphid Acyrthosiphon

Semipermeable species boundaries between Anopheles gambiaeand Anopheles arabiensis:. The role of introgressive. products were cloned by using pGEM-T Easy.A1360 pGEM®-T Easy Vector System I 20 réaction NA.51 PCR 1 202,00 15,00 171,70. A3610 pGEM®-T Vector System II 20 réaction NA.51 PCR 1 265,00 15,00 225,25.

Insertion polymorphism of transposable elements and. population insertion polymorphism vary for some TE. were cloned into a pGEM-T easy vector.

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The amplified fragment was cloned into pGEM-T Easy (Promega) forming pGEM-ITR. Genome-Wide Identification of Ampicillin Resistance Determinants in Enterococcus.